P3D Racks

Take advantage of the P3D culture and seeding racks to improve the usability of the P3D chamber.

Culture Rack

Culture P3D Rack

Seeding Rack

Three dimensional custom set-up
 

Seeding rack for the P3D chambers

Improving the results of the cell seeding process in porous scaffolds can be achieved by using a dynamic seeding technique. This procedure basically consists on perfunding a high cell concentration solution alternating the pumping direction at very slow flow rates, allowing the cells to get attached to the surface of the scaffold. This technique has been experimentally proved to provide highly homogeneous cell distributions as well as higher efficiencies compared to the manual seeding techniques.

EBERS has designed this rack to be used with the P3D chambers in dynamic seeding experimental set-ups. It allows the user to set up to five parallel seeding circuits with two chambers in each circuit and, just by easyly opening or closing the circuit stopcocks, using the same rack and circuit to culture the cells on the scaffolds after the seeding process.

  • Holds five independent parallel seeding circuits
  • Two P3D chambers in each circuit
  • Stainless-steel made, easy to clean
  • Build-up multiple experimental set-ups in a in a tydy way
  • Holds chambers and circuit reservoirs in vertical position, allowing for the visualization of medium levels
 

Technical Specifications

Culture Rack
Support rack for 5 seeding/culture circuits (5 x 2 chambers/circuit)
Chamber positions 10
Material Stainless steel
Compatibility P3D-6 and P3D-10 chambers
Autoclavable Yes
Cell culture atmosphere compatible Yes

Culture rack for P3D chambers

The P3D culture rack has been designed to keep up to five P3D chambers in vertical and tidy positions.

  • Fix the chambers in vertical position
  • Up to five chambers in parallel
  • Stainless-steel made, easy to clean
  • Build-up multiple experimental set-ups in a in a tydy way
 

Technical Specifications

Culture Rack
Support rack for P3D chambers
Chamber positions 5
Material Stainless steel
Compatibility P3D-6 and P3D-10 chambers
Autoclavable Yes
Cell culture atmosphere compatible Yes

Features

  • 3 parallel assays per chip
  • Optimal cell attachment
  • Optimized chip holder for easy manipulation
  • Fully integrated solution - pumping systems and accessories available
  • Coverslip-like bottom compatible with high-end microscopy
  • Small volume – minimal amount of medium andsupplement

Applications

  • Live cell imaging under flow-derived shear stress
  • Rolling and adhesion
  • Mimicking flow conditions in blood vessels
  • Long-term culture under mechanical stimulation of adherent cells

Physiochip configurations

  • Flow chip +

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  • Gradient chip +

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  • Transflow chip +

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