EBERS Medical Technology SL is devoted to the development of equipment for cell and tissue culture.
We are focused in creating new and innovative products which help the users to improve their protocols by providing new tools able to simplify research tasks or offering technical solutions which make easier to develop new experimental set-ups in simple and efficient way.
EBERS manufactures, markets and service products for research institutions in several countries by means of its distributors network and also provides extensive development services of bespoke culture chambers for tissue engineering.
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Tissue engineering is one of the cutting edge disciplines in biomedicine. Basically, it aims the development of tissues and organs by means of the application of in-vitro cell culture techniques in order to grow tissues, or even full organs, which can be used to replace those of the human body which are ill or have lost its functionality. Thus, tissue engineering promises a new paradigm in the treatment of many diseases as well as to revolution the current organ transplantation scheme since, when totally developed, full new organs will be grown in-vitro to replace those damaged removing the need from donors, which is maybe the bottleneck for the application of these techniques nowadays.
In the last decades, a lot of effort has been put in the investigation of the tissue engineering techniques, which pose a variety of challenges when compared to the traditional cell culture techniques. Perhaps the most relevant difference is related to the particular geometry of the substrates in which cells are grown aiming to develop an engineered tissue or organ. Usually, three dimensional scaffolds are used in tissue engineering, which makes a big difference compared with the usual bi-dimensional surface used in standard cell culture. The incorporation of three-dimensional scaffolds increases the complexity of the culture and difficults the oxygen and nutrients supply to the cells, causing cellular death when not performed properly.
Moreover the scaffold geometry, tissue engineering cultures are also strongly conditioned by the need of providing the cells with a growth environment which mimics the conditions which cell experience in-vivo as close as possible. This point is crucial for the cells to behave in a similarly to the in-vivo conditions and is of vital importance for the obtaining of "good quality" tissue. Tissue engineering bioreactors are designed to generate those culture conditions which, moreover the standard gas and temperature standard values, incorporate flow or mechanical stimulation conditions present in in-vivo systems.
EBERS develops tissue engineering bioreactors for research capable of simulating flow and direct substrate deformation conditions on different types of substrates and culture chambers suitable for porous and cylindrical scaffolds, membranes and sheet-like scaffolds. Also culture chambers, circuits and racks are available among our products in order to facilitate the labour of researchers. Moreover, we offer a technical service for the development and manufacturing of bespoke systems adapting to the particular requirements of your particular experimental set-up in order to satisfy the demands of our most demandings customers.
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One of the most important challenges of tissue engineering is the reproduction of an "artificial" vascular system able to supply the growing cells with sufficient oxygen and nutrients, as well as to remove the waste matter generated by cells metabolism, is one of the most difficult issues.
Cell cultures developed on three-dimensional scaffolds under static conditions are specially sensitive to oxygen and nutrients depletion. Assuming that they are equally distributed across the volume of the scaffold at the beginning of the culture, cells should develop at a similar rate in the whole scaffold. However, those cells located at the inner areas of the scaffold transform all the available oxygen and nutrients into carbon dioxide and waste matter soon, since renovation rate by diffusion is too slow compared to cell metabolism. Then, it typically happens that scaffolds larger than 1 mm result in a shell of viable cells and matrix surrounding a necrotic core due to the absence of vascular supply.
Thus, the improvement of mass transport and nutrient exchange in three-dimensional in-vitro experiments is crucial to get viable cells populations, for which the imposition of certain flow rate across the scaffold (perfusion) has been experimentally shown very efficient, compared to static cultures.
Likewise, flow stimulation is also crucial for the appropriate development of certain kinds of tissue as, for instance, the vascular tissue. In this case, endothelial cells in the intimal layer are strongly influence by the effect of blood flow, which has been experimentally demonstrated to exert a strong influence of the behaviour of such cells and, in general, of the whole vessel. Thus, culturing of blood vessels under flow conditions is of vital importance, since no other conditions better reproduce conditions experienced by cells in-vivo.
The TEB flow bioreactor series are versatile systems which allow setting up a wide variety of flow culture circuits for many different kinds of scaffolds. Check their capabilities of the two available models.
Thanks to its versatile configuration, EBERS bioreactors may be applied to develop a huge amount of experiments with different types of cells and scaffolds.
Here you will find some experiments which have been carried out in our laboratory using the EBERS equipments.