Cellusponge FAQ

  1. What is 3D CelluSponge made of?

    A: It is made of hydroxypropyl cellulose, a known biocompatible material, as the basic material which was side-chain modified for crosslinking purpose and ligand conjugation.

  2. Is 3D CelluSponge easy to use?

    A: Yes, the cell seeding is as easy as conventional 2D culture.

  3. How do I seed cells onto 3D CelluSponge?

    A: The cell seeding can be easily performed through absorption of cell suspension onto the surface of dry sponge. Firstly the cell suspension of desired cell density is dropped onto the dry sponge surface until the sponge absorbs the liquid completely. After 30-60 minutes of incubation, fresh pre-warmed culture medium can be added for extended cell culture maintenance. For further information, please kindly contact us and we will be pleased to advise you.

  4. Does 3D CelluSponge come ready to use?

    A: Yes, the sponge comes in your desired formats, pre-sterilized and ready for cell seeding experiments.

  5. Are there any published papers utilizing this sponge?

    A: Yes, there are some papers such as Yue et al. Biomaterials 2010, Gu et al. Regenerative Medicine 2010 & Nugraha et al. Biomaterials 2011.

  6. Does 3D CelluSponge come with well insert and or single unit?

    A: Yes, it comes in both formats. However we can also customize any formats based on your request and needs.

  7. Can the 3D cellusponge be re-used?

    A: We do not recommend re-using the 3D cellusponge. The 3D cellusponges are designed for single-use experiments.

  8. What cell types can I grow into spheroids using the 3D cellusponge?

    A: Our users have successfully demonstrated spheroid formation using many cell types.

  9. Why aren’t my cells forming spheroids?

    A: Not all cell types form spheroids readily when cultured in 3D cellusponge. Please check relevant literature to see if spheroid formation with your cell type of interest has been demonstrated before. Media composition is an important factor to spheroid formation, so make sure your culture media contains the necessary supplements.

  10. How long does spheroid formation take?

    A: Spheroid formation requires about 3-7days once inoculated. Air bubbles will be observed at time of inoculation. This will disappear with several days by the cells consuming the oxygen.

  11. Usually users grow cells for 3 to 4 days and at certain density, they subculture cells. How can they subculture in this sponge?

    A: Spheroids can be dissociated with Trypsin-EDTA (or TrypLE), counted and expanded into other culture vessels or into new sponges.

Cellusponge images and videos




Cellusponge product introduction.



Cellusponge cell seeding protocol.