The benefits of flow culture

One of the most important challenges of tissue engineering is the reproduction of an "artificial" vascular system able to supply the growing cells with sufficient oxygen and nutrients, as well as to remove the waste matter generated by cells metabolism, is one of the most difficult issues.

Cell cultures developed on three-dimensional scaffolds under static conditions are specially sensitive to oxygen and nutrients depletion. Assuming that they are equally distributed across the volume of the scaffold at the beginning of the culture, cells should develop at a similar rate in the whole scaffold. However, those cells located at the inner areas of the scaffold transform all the available oxygen and nutrients into carbon dioxide and waste matter soon, since renovation rate by diffusion is too slow compared to cell metabolism. Then, it typically happens that scaffolds larger than 1 mm result in a shell of viable cells and matrix surrounding a necrotic core due to the absence of vascular supply.

Thus, the improvement of mass transport and nutrient exchange in three-dimensional in-vitro experiments is crucial to get viable cells populations, for which the imposition of certain flow rate across the scaffold (perfusion) has been experimentally shown very efficient, compared to static cultures.

Likewise, flow stimulation is also crucial for the appropriate development of certain kinds of tissue as, for instance, the vascular tissue. In this case, endothelial cells in the intimal layer are strongly influence by the effect of blood flow, which has been experimentally demonstrated to exert a strong influence of the behaviour of such cells and, in general, of the whole vessel. Thus, culturing of blood vessels under flow conditions is of vital importance, since no other conditions better reproduce conditions experienced by cells in-vivo.

The TEB flow bioreactor series are versatile systems which allow setting up a wide variety of flow culture circuits for many different kinds of scaffolds. Check their capabilities of the two available models.



Flow culture products

  • TEB500 series +

    Improved accessibility for circuit manipulation and set-up! Read More
  • TEB1000 series +

    Two peristaltic pumping systems for high volume experimental set-ups. Read More
  • P3D chambers +

    Perfusion cell culture on porous scaffolds made easy. Read More
  • Tubular chambers +

    Control the rotation and luminal/external flow applied on your cylinder-shaped scaffolds. Read More
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TEB Series Facts!

  • Concept +

    The TEB series provide the conditions to develop cell culture under flow conditions in a simple versatile way. Read More
  • Architecture +

    TEB bioreactors are designed to reduce the contamination risk while while maximizing the useful space for experimental set-ups. Read More
  • Solved problems +

    Thanks to its smart design, the TEB series can solve many of the problems which arise when using home-made systems or other commercial solutions. Read More
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