Frequently Asked Questions

Here you will find some of the most frequently asked questions about the TC-3 bioreactor and its chambers.

Topics

If you cannot find the answers to your questions in this list, please contact us and will will attend you as soon as possible!

 

Culturing under load conditions

Tissue engineering aims producing tissues and organs in-vitro which can be used to replace those that are not working properly or are ill. There are a lot of factors which influence the result of such culture process and determine the successful obtaining of a functional "good quality" tissue. Among them, the type of scaffold and cells play a very important role, while the cell distribution after the seeding process, the oxygen and nutrients distribution through the volume of the scaffold and the scaffold microstructure have also a strong influence on the properties of the final tissue.

Moreover those factors, the conditions in which the culture is developed have also a crucial importance in the final result of a tissue engineered culture. The reason for this, is that most of the work in the construction of the new tissue is left to the action of cells seeded in the scaffold. Ideally, cells would proliferate in the scaffold expanding its number and generating new extracellular matrix to compose the new engineered organ or tissue.

However, it has been shown that cell behaviour -including the way they generate new tissue- is strongly influenced by the environmental conditions they are cultured, so the appropriate cells and scaffold culture in the wrong conditions can result in a non-valid or "bad quality" tissue, which is useless for the purposes it was created. Therefore, it is of crucial importance to carry out the cultures under to right conditions so cells generate quality tissue.

Then, the arising question is: which are the optimal conditions for cells to behave in the desired way? or, in other words, which are the culture conditions for cells to behave as they do when living in-vivo? And the obvious answer is that cells will behave the closer to the in-vivo conditions the closer the growing in-vitro conditions mimic the in-vivo conditions.

Among many other factors, it has been proved that cells strongly respond to mechanical stimuli and adapt their behaviour to the loading conditions, which is specially marked in some cases. The development of organs like bones, articular cartilage, blood vessels and many others, is influenced by the mechanical loading. Load tissue engineering bioreactors try to simulate the in-vivo growing conditions to some extent by applying direct loading on the culture substrate, which is of crucial importance in the obtaining of tissue with the desired properties.

The TC-3 tension/compression bioreactor is allows applying load on the cell culture substrate to reproduce the in-vivo conditions. Take a look at its features!

Subcategories

Flow culture products

  • TEB500 series +

    Improved accessibility for circuit manipulation and set-up! Read More
  • TEB1000 series +

    Two peristaltic pumping systems for high volume experimental set-ups. Read More
  • P3D chambers +

    Perfusion cell culture on porous scaffolds made easy. Read More
  • Tubular chambers +

    Control the rotation and luminal/external flow applied on your cylinder-shaped scaffolds. Read More
  • 1

TEB Series Facts!

  • Concept +

    The TEB series provide the conditions to develop cell culture under flow conditions in a simple versatile way. Read More
  • Architecture +

    TEB bioreactors are designed to reduce the contamination risk while while maximizing the useful space for experimental set-ups. Read More
  • Solved problems +

    Thanks to its smart design, the TEB series can solve many of the problems which arise when using home-made systems or other commercial solutions. Read More
  • 1