Obtaining an homogeneous cell growth in scaffolds becomes extremely difficult when scaffold are thicker than 2-3 mm. This phenomenon, which has been experimentally observed in a multitude of studies, is causes by the lack of nutrients and oxygen in the internal zones of the scaffold caused by cellular metabolic activity.
A feasible solution to this situation is based on the establishment of culture medium flow through the cultured scaffold (perfusion). By means of this technique the amount of nutrients and oxygen supplied to the cell living in the inner parts of the scaffold is improved, which is crucial for the development of those populations.
Cells can be grown on three-dimensional scaffolds in a wide number of ways. Using EBERS culture packages is a possible solution, while developing your own culture circuit might be the best option to satisfy the particular requirements of your experimental set-up.
Perfusion flow is crucial for the appropriate development of cells in thick scaffolds. EBERS perfusion chambers has been developed for that purpose and is suitable for different scaffold types and sizes as well as for multiple simultaneous experiments.