What is Go Matrix made of?
It is made of gelatin, a known biocompatible material
Is Go Matrix easy to use?
A: Yes, the cell seeding is as easy as conventional 2D culture.
How do I seed cells onto Go Matrix?
A: The cell seeding can be easily performed through absorption of cell suspension onto the surface of dry Go Matrix. Firstly the cell suspension of desired cell density is dropped onto the dry Go Matrix surface until the matrix absorbs the liquid completely. After 30-60 minutes of incubation, fresh pre-warmed culture medium can be added for extended cell culture maintenance. For further information, please kindly contact us and we will be pleased to advise you.
Can the Go Matrix be re-used?
A: We do not recommend re-using the Go Matrix. The Go Matrix is designed for single-use experiments.
What cell types can I grow into spheroids using the Go Matrix?
A: Our users have successfully demonstrated spheroid formation using many cell types.
Why aren’t my cells forming spheroids?
A: Not all cell types form spheroids readily when cultured in Go Matrix. Please check relevant literature to see if spheroid formation with your cell type of interest has been demonstrated before. Media composition is an important factor to spheroid formation, so make sure your culture media contains the necessary supplements.
How long does spheroid formation take?
A: Spheroid formation requires about 3-7days once inoculated. Air bubbles will be observed at time of inoculation. This will disappear within one day by the cells consuming the oxygen.
Usually users grow cells for 3 to 4 days and at certain density, they subculture cells. How can they subculture in this matrix?
A: Spheroids can be dissociated with Trypsin-EDTA (or TrypLE), counted and expanded into other culture vessels or into new Matrix.